A 1491 bp rbcs small subunit of ribulose bisphosphate carboxylase promoter was isolated from lemna gibba. Phytoextraction of zinc, copper, lead, iron, and nickel from landfill leachate by duckweed l. In order to address this issue, 25 pharmaceuticals, including 22 antibiotics, were assessed for phytotoxicity to the aquatic higher plant lemna gibba. With regard to the raw fiber is observed at a large quantity in l. Itis taxonomy is based on the latest scientific consensus available, and is provided as a general reference source for interested parties. Pdf aquatic microcosm assessment of the effects of. Abdallahoukarroum, 1 lotfibarhoumi, 2 mahshidsamadani, 1 anddaviddewez 1 d. It has a simple plant body, known as a thallus, which floats on the surface of the water and measures 3 5 mm in diameter. Lemna gibba, the gibbous duckweed, swollen duckweed, or fat duckweed, is a species of. Gibberellic acid ga3, indole3acetic acid iaa, salicylic acid sa, abscidic acid aba, jasmonic acid ja 1amino cyclopropane1carboxylic acid acc and aminoethoxyvinylglycine avg are popular growth regulators of plants. The test substances were applied in nine potency levels 17x, 18x, 21x24x, 28x, 30x, 33x and compared with controls unsuccussed and succussed water regarding their. The first draft genome of the aquatic model plant lemna minor. Nutrients and bioactive compounds of the lemna gibba.
They are morphologically divergent members of the arum family. Sulfamethoxazole smx is among the most frequently detected antibiotics in the environment, heavily used in both human therapy and agriculture. Lpipecolic acid was found to be effective in inducing flowering of lemna paucicostata 151, 381, 441 and 6746, and of lemna gibba g3. Boron is often present in water with high salinity. We developed efficient genetic transformation protocols for two species of duckweed, lemna gibba g3 and lemna minor 8627 and 8744, using agrobacteriummediated gene transfer. Lemna gibba and lemna minor in accordance with regulation eu no 20152283. When fed on duckweed alone, intake was low, feed conversion ratio good 1. All structured data from the file and property namespaces is available under the creative commons cc0 license. Duckweeda tiny plant with enormous potential for agriculture and environment lemna. While every effort has been made to provide the most reliable and uptodate information available, ultimate legal requirements with respect to species are contained in. Tillberg, stockholm were grown on k medium containing sucrose maeng and khudalri, 1973. For decades, these features made duckweeds an attractive model for. Nutrients and bioactive compounds of the lemna gibba and ulva.
Lemna gibba, in gibbous form, was always the stronger competitor, being able to overgrow and shade out lemna minor. Uptake was strongly regulated by the intracellular content of soluble sulfate. Photoperiodic requirements for flowering of the longday duckweed. This page was last edited on 12 october 2018, at 20.
Abstractthe effects of mercury hg, cadmium cd and chromium cr in concentrations ranging from 0. Oct 15, 2018 the surface floating duckweed lemna minor lemnaceae is a potential ingredient to replace the application of fishmeal in the aquafeed. However, the effects of their exogenous addition on the biomass production of aquatic plants, including lemnoideae plants, duckweeds, are largely unknown. The test substances were applied in nine potency levels 17x, 18x, 21x24x, 28x, 30x, 33x and compared with controls unsuccussed and succussed water regarding their influence on the plants growth rate. Fish were fed frequently on a diet containing 25% crude protein at a rate of23% of live body weight twice daily for 7 and 25 days. The results of both laboratory and field experiments have proved that the relative growth rate rgr of lemna gibba exceeds that of l. The 10 hr red photoperiod is also effective in inducing flowering when followed by a farred extension, but a red extension is ineffective.
At the onset of sulfate uptake the membrane potential was transiently depolarized. Comparison of lemna gibba and lemna minor from the. Lemna gibba in the germplasm resources information network grin, u. Introduction duckweeds are small, fastgrowing and morphologically simple. Lemna minor is a dark green plant which shines and its fronds are asymmetrical which are rounded without a point. Aquatic plants like eichhornia crassipes, azolla filiculoides, pistia stratiotes, hydrilla verticillata, typha domingensis, salvinia cucullata, azolla caroliniana, azolla pinnata, lemna minor, lemna aequinoctialis, lemna gibba, and spirodela polyrhiza are suitable aquatic plants for the removal of heavy metals as reported by several researchers. Frontiers a comparison of growth on mercuric chloride. However, it is highly toxic when the dosage surpasses the detoxification capability of an exposed organism, with involvement of an already described oxidative stress pathway. Test system stability and natural variability of a lemna gibba l. Research article toxic effects of nickel oxide bulk and. Three consecutive experiments were conducted in tanks 1.
The maximum accumulation of lead by lemna gibba occurs during the third day. Bioremediation the toxic effect of mercuryexposure in. Jun 12, 2018 excess boron b is toxic to aquatic organisms and humans. Growth and photosynthetic responses of lemna minor l. Frontiers mass production of lemna minor and its amino acid. These rapidly growing plants have found uses as a model system for studies in community ecology, basic plant biology, ecotoxicology, and production of biopharmaceuticals, and as a source of animal feeds for agriculture and aquaculture. The production capacity and competitive ability in two duckweed species lemna gibba l. Mean specific growth rates of lemna gibba in the laboratory and in mine tailing ponds. Aquatic microcosm assessment of the effects of tylosin on lemna gibba and myriophyllum spicatum. The present paper analyses the greentissue characteristics of a new l. This page was last edited on 18 october 2018, at 18. Status of the lemna gibba 7742a and lemna minor 8627 genomes. Test system stability and natural variability of a lemna.
The lemna protein concentrate is a source of natural and sustainable essential nutrients, like proteins, vitamins and minerals. Effects of metsulfuronmethyl on aquatic plant lemna gibba l. Toxic effects of nickel oxide bulk and nanoparticles on. Ivan baxter, purdue university, united states of america. Toxic effects of nickel oxide bulk and nanoparticles on the. Consequently, lemna gibba was exposed to smx to evaluate. By continuing to use our website, you are agreeing to our use of cookies. Potential of duckweed lemna minor for the phytoremediation. Effect of lpipecolic acid on flowering in lemna paucicostata. Research article toxic effects of nickel oxide bulk and nanoparticles on the aquatic plant lemna gibba l. Renault telmatophace arrhiza schur telmatophace generalis e. Lemna minor possesses small cells all over its surface ibid. Potential of lemna minor in ni and cr removal from aqueous. Duckweed lemna minor as a model plant system for the study.
In czechoslovakia, lemna gibba occurs mainly in nutrientrich fishponds, lowland oxbows and ditches. Download and install sejda desktop for mac and edit your pdf files offline. Frond area and frond number were measured with a commercial nondestructive image processing system at days 0. Partial purification and characterization of an insulinlike. Short term exposure of lemna minor and lemna gibba to mercury. Lemna minor, the socalled common duckweed, is a model system of. This study evaluated the effects of homeopathically potentized arsenicum album, nosode, and gibberellic acid in a bioassay with arsenicstressed duckweed lemna gibba l. Herbicidal effects of sulfamethoxazole in lemna gibba.
An illustrated flora of the northern united states, canada and the british possessions. Frontiers effect of exogenous general plant growth. History and sensitivity comparison of the spirodela polyrhiza. Lemna gibba and lemna minor are more commonly known as water lentils or duckweed. Pdf evolution of genome size in duckweeds lemnaceae. Lemna gibba freshwater macrophyte and seaweeds ulva lactuca of the middle basin papaloapan river, southeast of mexico were chemically characterized in their nutrients and bioactive compounds for possible use in the formulation of functional foods.
The allozyme study supports the continued recognization of two species and is concordant with the hypothesis that the species are related as progenitor and derivative. Jepson manual treatment for lemna gibba plants for a future arkive images of life on earth plantlife international the. Analysis of physicochemical characteristics of nanoparticles in solution indicated agglomerations of nionps in culture medium and a wide size. Ammonium uptake in lemna gibba g 1, related membrane. Consequently, lemna gibba was exposed to smx to evaluate phytotoxic potency and mode of action moa by hplcmsms measurement of paminobenzoic acid paba metabolite levels, a precursor to folate biosynthesis and substrate of the target enzyme dihydropteroate synthase dhps. Root elongation of the frond colonies in ms, b5 and white media is. Department of agriculture agricultural research service. Sulfate uptake into duckweed lemna gibba g1 was studied by means of 35ssulfate influx and measurements of electrical membrane potential.
Plants were exposed during 24 h to 0 mgl of nionps or niobulk. A read is counted each time someone views a publication summary such as the title, abstract, and list of authors, clicks on a figure, or views or downloads the fulltext. Like other sulfonamides, smx disrupts the folate biosynthetic pathway in bacteria, which was recently established as identical to that of plants, raising concerns over nontarget toxicity. Bioremediation the toxic effect of mercuryexposure in nile. Fusicoccin stimulated uptake up to 165% of the control even at ph 8. Effects of medium components and light on callus induction, growth, and frond regeneration in lemna gibba duckweed. She is the founder and editor of travel blog the tourist of life. The first draft genome of the aquatic model plant lemna.
In contrast to the mutants just described, there are other mutants of arabidopsis and lemna gibba where iaa homeostasis is lost, resulting in phenotypic abnormalities. Green tissuespecific analysis of a cloned rbcs promoter from. Duckweeds are of special interest, as they are naturally growing weeds that have the capacity to tolerate and remove toxic pollutants, including heavy metals from the environment. Files are available under licenses specified on their description page. Newsletter of the community of duckweed research and applications, edited by the iscdra lemna aequinoctialis 9075 spirodela polyrhiza 9509 lemna gibba g3 7741 lemna minor 5500 volume 4, issue, 2nd quarter 2016, pages 238 271 representatives for four species of duckweed are shown. Effects of media components and phytohormones on in vitro frond proliferation of lemna gibba g3 and 24 additional lemna gibba strains. Silver nanoparticle toxicity effect on growth and cellular viability of the aquatic plant lemna gibba abdallah oukarroum,y lotfi barhoumi,yzlaura pirastru,y and david dewezy ydepartment of chemistry, university of quebec in montreal, quebec, canada. To evaluate the potential of duckweed lemna minor l. Bioaccumulation and toxicity of lead pb in lemna gibba. Previously, researchers have used various lemnaceae species either to monitor andor remove heavy metals from freshwater systems.
Lemna gibba has relatively a low amount of distinct small cells on the outside and large cells in the middle. One mutant in arabidopsis, described by different laboratories under various names e. However, it is not a legal authority for statutory or regulatory purposes. Effect of the main operational variables such as ammonia concentration, residence time and lemna gibba to surface ratio on optimal conditions of ammonia removal from wastewater has been analyzed using the boxbehnken model design of experiments. We are very grateful to the editor that they managed the publication so. Stomp 1 institute of forest genetics, suwon kyonggido, republic of korea h. Flower induction, microscopeaided crosspollination, and seed production in the duckweed lemna gibba with discovery of a malesterile clone skip to main content thank you for visiting. Provided by ars systematic botany and mycology laboratory. Pdf comparison of lemna gibba and lemna minor from the.
For testing water quality or testing of chemicals with the duckweed growth inhibition test the two species lemna minor l. Effects of 25 pharmaceutical compounds to lemna gibba. In hybrid tilapia oreochromis niloticus x oreochromis aureus grown with duckweed lemna gibba or a combination of duckweed and commercial pellets, this combination gave the best performance. Cas9mediated genome editing in duckweeds, which will open new research avenues in using duckweeds for both basic and applied research. This species typically flowers during the longest days of summer. Studies have revealed that duckweed lemna minor can tolerate and remove heavy metals from aqueous solutions. Biochemical and standard toxic effects of acetaminophen on. Effects of homeopathic arsenicum album, nosode, and. Comparison of lemna gibba and lemna minor from the production. Newsletter of the community of duckweed research and. Metals have a variety of negative outcomes on plants, essential components of any ecosystem. To address the issue of the ecotoxicity of acetaminophen, we performed acute exposures of two aquatic plant species, lemna gibba and lemna minor, to this compound.
The aim of this study was to determine whether the growth performance of lemna gibba l. The vascular aquatic plant lemna gibba duckweed was exposed during seven days to lead pb at concentrations ranging from 50 to 300 mgl in a greenhouse with controlled photoperiod and temperature. Nutritive and bioactive compounds in lemna gibba and ulva lactuca. Material and methods lemna gibba strain g 3 stock cultures provided by dr. Duckweed research and applications status of the lemna gibba 7742a and lemna minor 8627 genomes evan ernst martienssen lab cold spring harbor laboratory, 1 bungtown rd, cold spring harbor, ny 11724 in 2009, cold spring harbor laboratory hosted a meeting funded by. We therefore investigated stability and natural variability of a lemna gibba bioassay under controlled laboratory conditions over about one year. As hg contamination is a pressing issue for aquatic systems worldwide, we assessed its impact on the growth of three commonly species of lemnaceae lemna gibba 6745, lemna minor 6580 and spirodela polyrhiza 5543. The proximate chemical analysis showed that ashes contents. Tracheophyta magnoliopsida lemnaceae lemna lemna gibba ecology this buoyant duckweed is a plant of still or slowly flowing, eutrophic water in ponds, canals, ditches or the quiet backwaters of rivers. We describe a novel model plant system for the study of human pathogenic bacterial infection on a large scale. Optimal conditions for the biological removal of ammonia from. It will generally not flower with 12 hours of darkness, such as at the equator or during the vernal equinox, because the nights are too long. This page was last edited on 23 august 2019, at 15. Lemna gibba was collected from the ponds around kermanshah and maintained in a nutrient medium.
You can change the display of the base map and layers by clicking on the layer control box in the upper righthand corner. This system was initiated by cocultivation of axenic duckweed lemna minor plants with pathogenic bacteria in 24well polystyrene cell culture plate. The culture technique of the duckweed was standardized in outdoor tanks and then applied in the pond. Pdf to narrow the differences between the results obtained from radionuclides and. The reduced morphology of lemna disperna and the allozyme data indicate that this species originated via dispersal of lemna gibba or. The roots of lemna gibba in 116 ms medium and 116 b5 are distinctly shorter than 116 white medium. Lemna gibba is a long day plant that flowers with 9 hours of darkness. Duckweed research and applications status of the lemna gibba 7742a and lemna minor 8627 genomes evan ernst martienssen lab cold spring harbor laboratory, 1 bungtown rd, cold spring harbor, ny 11724 in 2009, cold spring harbor laboratory hosted a meeting funded by the moore foundation to advance. All these results show the good nutritional value of species of the genus lemna and are prerequisite for their use in food rations of fish, birds, swine and other animals. Lemna gibba, a longday duckweed, can be induced to flower when the 10 hr white photoperiod is extended with red or farred light. Bioconcentration factor and removal efficiency were also calculated. Lemna gibba online atlas of the british and irish flora.
Usda, ars, germplasm resources information network. Flower induction, microscopeaided crosspollination, and. Nov 04, 2010 this study evaluated the effects of homeopathically potentized arsenicum album, nosode, and gibberellic acid in a bioassay with arsenicstressed duckweed lemna gibba l. The effects of pb on growth of lemna gibba were examined during days in the same experimental design. Partial purification and characterization of an insulinlike material from spinach and lemna gibba g3. The 10 hr red phot we use cookies to enhance your experience on our website. Open the online pdf editor with safari or your other favourite browser. Evidence for protonsulfate cotransport and its kinetics. Lemna gibba, the gibbous duckweed, swollen duckweed, or fat duckweed, is a species of lemna duckweed. Here, we report a case study on genome editing in a duckweed species.